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After removal and flushing with PBS, colon samples were cut into sections, pretreated to remove epithelial cells, then minced and digested with collagenase I, collagenase IV, and DNase to yield single-cell suspensions

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With the normal ageing process and wear and tear, these ligaments become enlarged (called ligamentum flavum) and bulky and like with the degenerate discs and facet joints, they reduce the space in the spinal canal

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