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RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency

However, just replacing three servings of meat a week with plant-based meat alternatives was not found sufficient to affect psychological measures

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All nutrients were irradiated to avoid pathogen contamination

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