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After cluster analysis of the obtained sequences, five groups obtained 1670, 1792, 1908 and 1921 representative bacterial communities OTUs, respectively

The targeted 3-PLG-ibu-A-GSH and ibuprofen at higher, 1.5 M concentration (the estimated drug content of the 3-PLG-ibu-A-GSH nanocarriers) significantly protected against the barrier damaging effect of cytokines with the ibuprofen-coupled targeted nanocarrier group showing the highest effect against BBB dysfunction (Fig

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The expression levels for each of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM) using a software package called RSEM 79

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