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Using 5-chloromethylfluorescein diacetate (CMFDA), which has been reported to be a potent and irreversible GSTO1 inhibitor 22 , we optimized a gel-based binding assay that measures competitive inhibition of CMFDA binding to recombinant GSTO1 or endogenous GSTO1 in a soluble proteome (Supplementary Fig