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Total cell lysates were prepared using lysis buffer (20 mM Hepes (pH 7.4), 150 mM NaCl, 50 mM KF, 50 mM -glycerolphosphate, 2 mM EGTA (pH 8), 1 mM Na 3 VO 4 , 1% Triton X-100, 10% glycerol and 1 protease inhibitor cocktail (Calbiochem))

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110 However, treatment with A-485 lowers H3K18la levels and suppresses osteogenic gene expression in BMSCs, thereby exacerbating osteoporosis

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Glutathione is produced in the liver and is made up of the three amino acids glycine, cysteine, and glutamic acid