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Soto, M

For quantification of sulfur-containing metabolites in cultured cancer cells and mouse serum, 10 l samples were extracted with 90 l of ice-cold extraction solvent (80% methanol and 20% H 2 O) containing isotope-labelled internal standards (36 M 13 C 2 15 N-GSH, 0.92 M 13 C 4 15 N 2 -GSSG and 10 M D 4 -cystine)

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MRPs prepared from fermentation of milk proteins by lactic acid bacteria show higher antioxidant activity than the intact milk protein, low intracellular ROS production and sustain reduced-GSH levels in hepatocytes 630

Notably, the concentration-dependent effects of ROS on glycolysis are distinct