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The peptide adopts a structural configuration in which the histidine imidazole nitrogen, the glycine amino terminus, and the deprotonated amide nitrogen of the glycine-histidine peptide bond all participate in coordinating a divalent copper ion

and -actin PCRs with primers: forward 5-CCAAGGCCAACCGCGAGAAGATGAC-3, reverse 5-AGGGTACATGGTGGTGCCGCCAGAC-3