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Following a 2 h incubation at 37 C, culture medium was removed, and 75 l of DMSO was added per well, after which absorbance at 540 nm was measured with a Synergy H1 microplate reader (BioTek Instruments, Inc.)

When you are ready to switch, locate the small capsule bead inside the filter (you can feel it between your fingers) and press firmly until you hear or feel a click

stomach ache, headache and weakness) and nine complained of some degree of throat irritation 34

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