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doi: 10.1210/jc.2014-1425 253

Membranes were blocked with 5% non-fat milk or BSA in TBST and then incubated overnight at 4 C with the appropriate primary antibodies

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To investigate the changes in the microbiota and its association with gallstone formation, researchers have utilized 16S rRNA gene sequencing to analyze microbial components in bile, gut, and gallstones of patients with cholelithiasis (Shen et al., 2015

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